Separation and Purification of Polysaccharides Sulfate from Ulva lactuca and Its Structure Analysis
- VernacularTitle:石莼硫酸多糖的分离纯化及结构解析
- Author:
Chuanchi ZHENG
;
Qianru ZHANG
;
Qunfang LI
- Publication Type:Journal Article
- Keywords:
Ulva lactuca;
Polysaccharides sulfate;
Phenyl-sulfuric acid method;
HPGC;
IR;
NMR spectrum
- From:
China Pharmacy
2007;0(27):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the separation and purification of polysaccharides sulfate from Ulva lactuca,and to analyze its structure.METHODS:The separation and purification method of polysaccharides sulfate from U.lactuca was optimized.The content of polysaccharides in polysaccharides sulfate was determined by phenyl-sulfuric acid method.The molecular amount of polysaccharides was determined by HPGC.The structure of polysaccharides was analyzed by IR and 13C-NMR.RESULTS:The optimal extraction condition of polysaccharides sulfate from U.lactuca were crashed materials 20 mesh sieve,20-fold water,decocting 3 times at 85 ℃,2 hours each time.The content of polysaccharides was 22.64% and molecular amount was 1 156 112.Soluble polysaccharides mainly contained ?-glucuronic acid,rhamnose and plenty of sulfate radical.CONCLUSION:The method is simple and stable,which provides theoretic basis for production of polysaccharides sulfate from U.lactuca.
