Content Determination of Berberine in Fujie Lotion by RP-HPLC
- VernacularTitle:RP-HPLC法测定妇洁洗剂中小檗碱的含量
- Author:
Rongzhi LU
;
Yunjing LI
;
Xueqin LI
- Publication Type:Journal Article
- Keywords:
Fujie lotion;
Berberine;
RP-HPLC;
Content determination
- From:
China Pharmacy
2007;0(27):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop an RP-HPLC method for the content determination of berberine in Fujie lotion.METHODS:The separation was performed on Agilent 1200 technologies XDB-C18(250 mm?4.6 mm,5?m)column with acetonitrile-0.02%monobasic potassium phosphate(45:55)as mobile phase at flow rate of 1.0 mL?min-1.The UV detection wavelength was set at 265nm.RESULTS:The linear range of berberine were 0.029 6～0.236 8?g(r=0.999 1)with an average recovery of 99.21%(RSD=1.10%,n=9).CONCLUSION:Established method is sensitive,simple,rapid,accurate and reproducible for the quality control of Fujie lotion.
