Study on HPLC Fingerprint of Desmodium styracifolium and Its Preparations
- VernacularTitle:广金钱草及其制剂的HPLC指纹图谱研究
- Author:
Shengguo JI
;
Nianxin GUO
;
Yingchun LI
;
Dong WANG
- Publication Type:Journal Article
- Keywords:
Desmodium styracifolium;
Fingerprint;
RP-HPLC
- From:
China Pharmacy
2007;0(27):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish RP-HPLC fingerprints of Desmodium styracifolium and its preparations in order to provide basis for the quality evaluation of them.METHODS:The separation was performed on ODS-C18(250 mm?4.6 mm,5 ?m) column with mobile phase consisted of methanol-20% phosphoric acid(gradient elution).The detection wavelength was set at 360 nm and flow rate was 1.0 mL?min-1.Column temperature was set at 25 ℃.The RP-HPLC fingerprint of D.styracifolium and its preparations were recorded.RESULTS:There were 13 common peak in RP-HPLC fingerprint of D.styracifolium and its preparations.CONCLUSION:The method is accurate,stable and reproducible for basis of quality evaluation and RP-HPLC finger print of D.styracifolium and its preparations.
