Content Determination of Praeruptorin A in Qingfei Yihuo Pills by RP-HPLC
- VernacularTitle:RP-HPLC法测定清肺抑火丸中白花前胡甲素的含量
- Author:
Ruihong HUANG
- Publication Type:Journal Article
- Keywords:
Qingfei yihuo pills;
Praeruptorin A;
RP-HPLC;
Content determination
- From:
China Pharmacy
2007;0(27):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish RP-HPLC method for the content determination of praeruptorin A in Qingfei yihuo pills.METHODS:The chromatographic column was Diamonsil-C18(250 mm?4.6 mm,5 ?m) column.The mobile phase consisted of methanol-water(81:19) and the detection wavelength were set at 322 nm.RESULTS:The linear range of praeruptorin A was 4.65～23.25 ?g?mL-1(r=0.999 9) with an average recovery of 100.5%(RSD=2.35%,n=6).CONCLUSION:The method is sensitive,well separated and accurate for content determination of Qingfei yihuo pills.
