Improvement of Determination Method of Xiao'er Resuqing Oral Solution in Chinese Pharmacopiea(2005 edition)
- VernacularTitle:对2005年版《中国药典》小儿热速清口服液中含量测定方法的改进
- Author:
Nong ZHOU
;
Yin WANG
;
Conglong XIA
- Publication Type:Journal Article
- Keywords:
Xiao'er resuqing oral solution;
Content determination;
Baicalin;
HPLC;
Improvement
- From:
China Pharmacy
2007;0(27):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To improve the determination method of Xiao'er resuqing oral solution in Chinese Pharmacopeia(2005 edition).METHODS:HPLC method was used to determine the content of baicalin.The separation was performed on Agilent zorbax SB-C18(150 mm?4.6 mm,5 ?m) column with methanol-water-phosphoric acid(45:55:0.2) as the mobile phase at flow rate of 1.0 mL?min-1.The detection wavelength was set at 276 nm.RESULTS:The linear range of baicalin was 0.5～100 ?g?mL-1(r=0.999 9) with an average recovery of 99.83%(RSD=0.99%,n=9).CONCLUSION:The method is rapid,convenient and accurate,and can be used as reference evidence for the content determination of Xiao'er resuqing oral solution in Chinese Pharmacopoeia(2005 edition).
