Determination of Psoralen and Isopsoralen in Laonian Kechuan Tablets by RP-HPLC
- VernacularTitle:RP-HPLC法测定老年咳喘片中补骨脂素和异补骨脂素的含量
- Author:
Jianming QIN
- Publication Type:Journal Article
- Keywords:
Laonian kechuan tablets;
Psoralen;
Isopsoralen;
RP-HPLC;
Content determination
- From:
China Pharmacy
2007;0(27):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for content determination of psoralen and isopsoralen in Laonian kechuan tablets.METHODS:RP-HPLC was applied.The separation was performed on Kromasil C18(250 mm?4.6 mm,5 ?m) column with mobile phase of methanol-water(55:45) at flow rate of 1.0 mL?min-1.The detection wavelength was set at 246 nm.RESULTS:The linear range of psoralen were 0.047 0～0.940 0 ?g with an average recovery of 97.70%(RSD=0.89%,n=9).The linear range of isopsoralen were 0.046 0～0.920 0 ?g with an average recovery of 98.76%(RSD=0.84%,n=9).CONCLUSION:The method is simple,rapid and accurate for the quality control of Laonian kechuan tablets.
