Effects of immune-activated platelets and LDL on expression and activity of COX-2 and PPAR-? in HUVECs
- VernacularTitle:免疫活化血小板对人脐静脉内皮细胞COX-2及PPAR-?表达与活性的影响及LDL的作用
- Author:
Yanqing WU
;
Xiaoshu CHEN
;
Junbing CHAI
- Publication Type:Journal Article
- Keywords:
Immune-activated platelets;
Atherosclerosis;
Cyclooxygenase-2;
Peroxisome proliferator activated receptor-?;
Human umbilical vein endothelial cells
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effect of immune-activated platelets and low-density lipoprotein cholesterol (LDL) on the expression and activity of cyclooxygenase-2 (COX-2) and peroxisome proliferator activated receptor ? (PPAR-?) in human umbilical vein endothelial cells (HUVECs) treated with activated platelets and LDL.METHODS:The platelets were activated by ADP.The co-culture system of HUVECs with immune activated platelets and/or LDL were established.The activity of COX-2 and expression of PPAR-? at mRNA and protein levels in HUVECs were detected by RT-PCR and Western blotting.The concentration of PGE2 was measured by ELISA for representing the COX-2 activity.The PPAR-? activity was determined by a nuclear factor assay kit.RESULTS:The COX-2 activity and mRNA expression of PPAR-?,the protein levels of COX-2 and PPAR-? and PGE2 concentration in activated platelets group were significant higher than those in un-activated platelets group (all P
