Protective effects of riboflavin on diabetic nephropathy in STZ-induced diabetic rats
- VernacularTitle:核黄素对STZ诱导的大鼠糖尿病肾病的治疗作用
- Author:
Guoguang WANG
;
Cui ZHANG
;
Xiaohua LU
;
Wei LI
- Publication Type:Journal Article
- Keywords:
Riboflavin;
Diabetic nephropathies;
Transforming growth factor beta;
Plasminogen activator inhibitor-1
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To explore the protective effects of riboflavin on the kidney in streptozotocin (STZ)-induced diabetic rats.METHODS:Male Sprague-Dawley rats were randomly divided into 3 groups:normal control group,diabetic model group and riboflavin-treated group.Diabetes was induced by a single injection of STZ (dissolved in 0.01 mol/L citrate buffer,pH 4.5,65 mg/kg,ip) in rats.The biochemical methods were used to measure the contents of urine protein and malondialdehyde in the kidney,and the activities of superoxide dismutase (SOD) and catalase (CAT) in serum and renal tissues.Furthermore,the protein expression of TGF-?1 and plasminogen activator inhibitor-1(PAI-1) in renal cortex was detected by Western blotting.The morphological changes of renal tissue were observed under microscope.RESULTS:Compared to the diabetic model group,riboflavin significantly increased the activities of SOD and CAT (P
