Down-regulation of GEP100 inhibits the invasive ability of pancreatic cancer cell AsPC-1 in vitro
- VernacularTitle:GEP100基因沉默抑制胰腺癌细胞AsPC-1的侵袭能力
- Author:
Chuangao XIE
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
E-cadherin;
Neoplasm invasiveness;
Neoplasm metastasis;
Guanine nucleotide exchange protein 100
- From:
Chinese Journal of Pathophysiology
2000;0(07):-
- CountryChina
- Language:Chinese
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Abstract:
AIM:To study the effects of down-regulation of guanine nucleotide exchange protein 100 (GEP100) on the invasive ability of pancreatic cancer cell AsPC-1 in vitro.METHODS:The clone of AsPC-1 cells with stable knock-down of GEP100 by transfection of pSuper-retro-puro-GEP100 was established.The invasive ability was evaluated by matrigel invasion assay and the migratory ability of the cells was examined by crossing-river test.The protein expression of E-cadherin was determined by Western blotting.RESULTS:The invasive ability was inhibited significantly in matrigel invasion assay (P
