Content Determination of Oleanolic Acid and Ursolic Acid in Prunella vulgaris by HPLC
- VernacularTitle:HPLC法同时测定夏枯草中齐墩果酸和熊果酸的含量
- Author:
Shuying GUO
;
Bo FENG
- Publication Type:Journal Article
- Keywords:
HPLC;
?-CD;
Oleanolic acid;
Ursolic acid;
Content determination;
Prunella vulgaris
- From:
China Pharmacy
2007;0(27):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC method for the content determination of oleanolic acid and ursolic acid in Prunella vulgris with ?-CD as additive agent of mobile phase.METHODS:The sample was Prunella vulgaris.The separation was performed on a Tigerkin ODS3(250 mm?4.6 mm,5 ?m) column with mobile phase consisted of methanol-10 mmol?L-1 ?-CD 1% ammonium acetate(86:14) at flow rate of 1.0 mL?min-1.The column temperature was set at 40 ℃ and UV detection wavelength was 210 nm.RESULTS:The linear range of oleanolic acid and ursolic acid were 0.5～5.0 ?g(r=0.999 2) and 1.0～10.0 ?g(r=0.999 8) respectively.High,medium and low average recovery of oleanolic acid were 97.3%(RSD=1.04%),98.0%(RSD=0.87%),101.6%(RSD=1.65%),respectively.Those of ursolic acid were 97.8%(RSD=1.17%),98.7%(RSD=0.93%),100.4%(RSD=1.72%),respectively.CONCLUSION:The new method is simple,accurate and reproducible for the quality control of P.vulgaris and simultaneous determination of oleanolic acid and ursolic acid.
