Effect of Artemisia Argyi Extracting Solution Contained Rat Serum on Eexpression of Transforming Growth Factor-?_1 and Protein Smads in Hepatic Stellate Cells
- VernacularTitle:蕲艾提取液含药血清对肝星状细胞转化生长因子?_1及Smad蛋白表达的影响
- Author:
Zhenfang XIONG
;
Weimin WANG
;
Xinying FEI
;
Chizhi ZHANG
- Publication Type:Journal Article
- Keywords:
Artemisia Argyi extracting solution;
Hepatic stellate cell;
Transforming growth factor-?1;
Smads protein;
Hepatic fibrosis
- From:
Journal of Traditional Chinese Medicine
1993;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect and mechanism of Artemisia Argyi extracting solution on anti-hepatic fibrosis.Methods Twenty-four experimental Wistar rats were randomized into four groups:Artemisia group,Danshen group,physiological saline group,and blank control group,with 6 in each.The Artemisia group was treated with 20% Artemisia Argyi extracting solution,the Danshen group with Danshen (salvia) solution,and the physiological saline group with physiological saline by gavage,20ml/kg.After treating three times per day for 3 days,sera were obtained and made a mixture with DMEM to be volume fraction as 5%,10%,and 20%.Effect of different volume fraction of extracting solution on TGF-?1 Smad3 and Smad7 mRNA and protein were detected.Results After treating with 5%,10% and 20% Artemisia Argyi extracting solution contained rat serum for 24h,contrasted to the black control group,the expression of TGF-?1 mRNA level was (0.82?0.03),(0.72?0.03) and (0.67?0.04) times,and the expression of TGF-?1 protein was (0.66?0.09),(0.34?0.05) and (0.31?0.07) times respectively;contrasted to the physiological saline group,the expressions of TGF-?1 mRNA and protein were significantly decreased (P
