Study on Making the Animal Model of Diarrhea-predominant Irritable Bowel Syndrome Due to Liver-Spleen Disharmony
- VernacularTitle:肝脾不调所致腹泻型肠易激综合征的动物模型研究
- Author:
Rui HU
;
Fang TANG
- Publication Type:Journal Article
- Keywords:
Diarrhea;
Irritable bowel syndrome;
Animal model;
5-hydroxytryptamin;
Lactate dehydrogenase;
Aquaporin
- From:
Journal of Traditional Chinese Medicine
1993;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the animal model making method for diarrhea-predominant irritable bowel syndrome (IBS-D) caused by the disharmony of liver and spleen in TCM.Methods In order to define the period of the animal model making,the 32 neonatal rats were divided into 3 days group,6 days group,9 days group and control group.In order to determine the weight of the experimental rats,the 48 rats with different weight were divided into lower weight model group,lower weight control group,medium weight model group,medium weight control group,higher weight model group and higher weight control group.In order to decide the method of causing diarrhea,the 32 rats were divided into lactose enriched diet group,restraint stress group,lactose enriched diet plus restraint stress group,and a control group.The body weight and diarrhea rate of all groups were compared respectively.After the method of model making was decided,the 16 rats were divided into a model group and a control group,and 5-hydroxytryptamin (5-HT),lactate dehydrogenase (LDH) and aquaporin 4 (AQP4) of the two groups were compared for model-proving.Results Based on the results step by step,the best method was decided:the period of the animal model making was 6 days,rats with higher body weight should be subjects,and the lactose enriched diet combining with restraint stress should be used to induce diarrhea.Model-proving test:compared with the control group,5-HT and LDH decreased (P
