Study on Protective Effect of Astragaloside on Nerve of in Vitro and in Vivo Model of Parkinson Disease
- VernacularTitle:黄芪甲苷对帕金森病体外、体内模型的神经保护作用研究
- Author:
Lixia XIE
;
Hongle LIU
;
Zhihong XIA
;
Ying WU
- Publication Type:Journal Article
- Keywords:
Parkinson disease;
Astragaloside;
Nerve protection;
PC12 cell;
MPTP
- From:
China Pharmacy
2005;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To study the protective effect of astragaloside on nerve of in vitro and in vivo model of Parkinson disease (PD).METHODS: MPP+ induced PC12 cell injury to establish in vitro PD model.PD mice model was induced by MPTP.MTT assay was used to determine the effect of astragaloside on survival rate of PC12 cell induced by MPP+ and the content of LDH and MDA.The effect of astragaloside on spontaneous behavior and the content of striatal DA and HVA were also detected.RESULTS: 25 ?mol?L-1,50 ?mol?L-1,100 ?mol?L-1 dose of astragaloside inhibited the decrease of survival rate of PC12 cell induced by MPP+ in dose dependent manner.10 ?mol?L-1,20 ?mol?L-1,40 ?mol?L-1 dose of astragaloside can obviously enhanced spontaneous behavior of model mice,and reduced the content of striatal DA and HVA.CONCLUSION: Astragaloside can protect nerve of in vivo PD and in vitro PD model.
