Extraction and Identification of Effective Constituent of Magnolia officinalis
- VernacularTitle:厚朴有效成分的提取分离与结构鉴定
- Author:
Qiuming YANG
;
Meizhen CHEN
- Publication Type:Journal Article
- Keywords:
Magnoliae offcinalis;
Effective constituent;
Extraction;
Separation;
RP-HPLC;
Identification
- From:
China Pharmacy
2005;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To extract effective constituent of Magnolia offcinalis and to identify its structure.METHODS: Ultrasound extraction was used to extract magnolol and honokiol from M.officinalis and the extracts were isolated using polyamide column chromatography.The content of magnolol and honokiol were determined using RP-HPLC.RESULTS: The optimal extractive condition was moderate ultrasonic,95% alcohol,8-fold solvent,extracting for three times,each time for 30 min.The extraction rates of phenols,magnolol and honokiol were 1.92%,1.26% and 0.66%,respectively.Honokiol was obtained by polyamide column chromatography.CONCLUSION: The method is simple,stable and supply reference for the extraction and identification of M.officinalis.