Simultaneous Determination of Peoniflorin, Campanulin and Ferulic Acid in Kangmin Granules by HPLC
- VernacularTitle:HPLC法同时测定抗敏颗粒中芍药苷、毛蕊异黄酮苷和阿魏酸的含量
- Author:
Gaijin XUE
;
Yunli ZHAO
;
Jie HAO
;
Xiaoxia GAO
;
Zhiguo YU
- Publication Type:Journal Article
- Keywords:
Kangmin granules;
HPLC;
Peoniflorin;
Calycosin;
Ferulic acid;
Content
- From:
China Pharmacy
2005;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC method for simultaneous determination of peoniflorin,calycosin and ferulic acid in Kangmin granules.METHODS: The separation was performed on a Kromasil C18 column (250 mm?4.6 mm,5 ?m),and the mobile phase consisted of methanol-acetonitrile-0.4% phosphonic acid (20 ∶ 10 ∶ 70) with flow rate of 1.0 mL?min-1.The detection was performed at wavelength of 254 nm.Column temperature was room temperature and the injection volume was 10 ?L.RESULTS: The linear ranges of peoniflorin,calycosin and ferulic acid were 180～3 600 ?g?mL-1(r=0.999 2),4.6～92.0 ?g?mL-1(r=0.999 1) and 8.0～160.0 ?g?mL-1(r=0.999 4),respectively.The average recoveries were 98.3%(RSD=1.8%,n=9),99.0% (RSD=2.0%,n=9) and 100.5%(RSD=1.1%,n=9),respectively.CONCLUSION: The method is simple,accurate,sensitive and reproducible for the quality control of Kangmin granules.
