Study on HPLC Fingerprint of Compound Danshen Tablets
- VernacularTitle:复方丹参片的HPLC指纹图谱研究
- Author:
Ying WANG
- Publication Type:Journal Article
- Keywords:
Compound danshen tablets;
HPLC;
Fingerprint;
Chemical component classification
- From:
China Pharmacy
2005;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish HPLC fingerprint of Compound danshen tablets.METHODS: The separation was performed on Agilent Eclipse XDB-C18(150 mm?4.6 mm,5?m) column with mobile phase consisted of acetonitrile-0.04% phosphate (gradient elution) at flow rate of 1.0 mL?min-1.Detection wavelength was set at 272 nm and column temperature was 30℃.RESULTS: Established HPLC fingerprint was distinct and reproducible.There were 8 characteristic peaks among which No.5 characteristic peaks,No.6,No.7,No.8 came from HPLC fingerprint of Salvia miltiorrhiza and No.1 characteristic peaks,No.2,No.3,No.4 came from HPLC fingerprint of Panax notoginseng.CONCLUSION: The characteristic peaks of effective parts of Compound danshen tablet mainly come from S.miltiorrhiza.The study can be used for tracing and isolation of effective part of Compound danshen tablet and establishes basis for the further development of Chinese patent drugs.
