Content Determination of Gentiopicroside in Xiaofeng Pills by RP-HPLC
- VernacularTitle:RP-HPLC法测定消风丸中龙胆苦苷的含量
- Author:
Xiuhua YU
;
Chun FU
- Publication Type:Journal Article
- Keywords:
RP-HPLC;
Xiaofeng pills;
Gentiopicroside;
Content determination
- From:
China Pharmacy
2005;0(23):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a RP-HPLC method for the content determination of gentiopicroside in Xiaofeng pills.METHODS: The chromatographic column was DiamonsiLTM C18(150 mm?4.6 mm,5 ?m) column with mobile phase consisted of acetonitrile-water(11 ∶ 89) at a flow rate of 1.0 mL?min-1.The detection wavelength was set at 254 nm.RESULTS: The linear range of gentiopicroside were 0.205 6～2.056 0 ?g (r=0.999 9) with an average recovery of 96.18%(RSD=1.55%,n=6).CONCLUSION: The method is simple,rapid and accurate for the quality control of Xiaofeng pills.
