Effects of endothelial nitric oxide synthase on zero stress state of carotid arteries in rats of different ages
- VernacularTitle:不同月龄成年大鼠颈动脉零应力状态与内皮型一氧化氮合酶的关系
- Author:
Zhihong LI
;
Zhiming WANG
;
Li FANG
;
Hua GAO
- Publication Type:Journal Article
- Keywords:
Arteries;
Vascular Remodeling;
Residual Stress;
Opening Angle;
Rats
- From:
Chinese Journal of General Surgery
1997;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the distribution and effect of endothelial nitric oxide synthase (eNOS) on the zero-stress state of carotid arteries in rats at different ages.Methods Morphometry and microstructure of carotid artery in 3-month-old,6-month-old and 12-month-old Sprague-Dawley rats were studied quantitatively by histological method and computer image analysis.Immunohistochemistry and the NADPH-diaphorase reaction were used to localize the endothelial nitric oxide synthas (eNOS) in different age rats.The changes of opening angle in the zero-stress state was also observed.Results With increasing age,there was a significant reconstruction changes in morphometric parameters and microstructure parameters of the carotid arteries in rats.While the expression of eNOS protein of endothelium and vascular smooth muscle cells were decreased.Compared with 3-month-old rats,the zero-stress state.of opening angle in 6-month-old and 12-month-old group were increased.However,pre-incubation of vessel rings with L-arginine (30 mmol/L),a substrate of eNOS,attenuated the changes of the zero-stress state.Conclusions The results suggest that the carotid arterial wall in different age rats shows unequal growth and different zero-stress state eNOS may be involved in the age-related changes of zero-stress state of the carotid artery in aged rats.The results may provid an experimental base for vascular transplantation and vascular reconstruction.
