Biological characteristics and induced differentiation of bone marrow-derived mesenchymal stem cells from sensitized mice in vitro
- VernacularTitle:致敏小鼠骨髓间充质干细胞体外培养及多向分化功能的测定
- Author:
Dongling HONG
;
Yanfeng WU
;
Lhong XU
;
Jianpei FANG
- Publication Type:Journal Article
- Keywords:
Sensitized mice;
Mesenchymal stem cells;
Cell differentiation
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To evaluate the biological characteristics and differentiating potentials of bone marrow-derived mesenchymal stem cells(MSCs) from sensitized mice by allogeneic splenocyte transfusion in vitro.METHODS: Adherent culture method was applied for culturing the bone marrow-derived MSCs from sensitized mice.The cell morphology was examined and the surface marker profiles were analyzed by flow cytometry.The differentiating potentials of the MSCs into osteogenic,adipogenic and myogenic lineages were explored.The bone marrow-derived MSCs from the normal mice were collected and served as controls.RESULTS: Both the bone marrow-derived MSCs from sensitized and normal mice were exhibited a homogeneous distinctive morphology and were positive for the surface markers CD29,CD105,CD44 and Sca-1,negative in CD 34 and CD11b.The abilities of both MSCs to differentiate into osteogenic,adipogenic and myogenic pathways in the same condition were also observed.CONCLUSION: There is no difference in the biological characteristics and induced differentiating potentials between the sensitized mouse bone marrow-derived MSCs by allogeneic splenocytes transfusion and the MSCs from normal mice.
