Effects of zanthoxylum seed oil_(A2) on NF-?B signaling pathway and inflammatory factor in lung tissue of asthmatic mice
- VernacularTitle:椒目油_(A2)对不同时相哮喘小鼠肺组织NF-?B信号通路及炎症因子表达的影响
- Author:
Junqin WANG
;
Fucheng MA
;
Weibin TIAN
;
Shengchun WANG
;
Huiping ZHAO
- Publication Type:Journal Article
- Keywords:
Zanthoxylum seed oil;
Asthma;
NF-kappa B;
Interlukin-5;
Intercellular adhesion molecule-1
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the dynamic influence of zanthoxylum seed oilA2(ZSOA2) on NF-?B signaling pathway and inflammatory factor in pulmonary tissue of asthmatic mice.METHODS: The suspensoid(0.2 mL containing 20% albumin hydroxide and 10% ovalbumin) was administered by intraperitoneal injection to sensitize the BALB/c mice on day 1,then 0.4% ovalbumin solution(50 ?L in phosphate buffer fluid) was dripped into the respiratory tract through nasal cavity to establish the asthmatic mouse model.After dripped ovalbumin for 24 h,48 h,3 d,7 d and 14 d,the mice were killed at specified time points.The contents of interleukin-4(IL-4),interleukin-5(IL-5) and interferon-?(IFN-?) in bronchoalveolar lavage fluid(BALF) were determined by ELISA.The pathological changes of the lung tissues were observed with HE staining.The inflammatory cell counts were conducted by Eosin staining.The protein levels of adhesion molecule and the molecules of NF-?B signaling pathway in lung tissues were determined by Western blotting.RESULTS: In ZSOA2 treated mice,the pathological injury of the lung was significantly attenuated as compared to the model mice,the counts of eosinophils and lymphocytes were reduced obviously in lung bronchial area of asthmatic mice at all observed time points(P
