Therapeutic mechanisms of tumor necrosis factor-? monoclonal antibody on hepatopulmonary syndrome in rats
- VernacularTitle:TNF-?单克隆抗体治疗大鼠肝肺综合征的机制研究
- Author:
Li LIU
;
Zhi ZHAO
;
Nan LIU
;
Yali WU
;
Jie FU
- Publication Type:Journal Article
- Keywords:
Tumor necrosis factor-? monoclonal antibody;
Tumor necrosis factor;
Inducible nitric oxide synthase;
Hepatopulmonary syndrome
- From:
Chinese Journal of Pathophysiology
1989;0(06):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the expression of iNOS in the lung of HPS rats treated with tumor necrosis factor-? monoclonal antibody(TNF-?-McAb) and to investigate the therapeutic mechanism of TNF-?-McAb on hepatopulmonary syndrome.METHODS: Male Sprague-Dawley rats,weighing(250?25)g,were randomized to sham operation group,common bile duct ligation(CBDL) group and CBDL+TNF-? McAb treatment group.Histopathological changes of the lung tissue were evaluated by hematoxylin and eosin staining.The mRNA expression of iNOS in the lungs of hepatopulmonary rats was examined by RT-PCR,while the changes of iNOS in the protein level were evaluated by immunohistochemistry and Western blotting.RESULTS: The inflammatory responses in the CBDL rats treated with TNF-?-McAb decreased than that in CBDL group.Compared to CBDL group,the distribution of iNOS protein and the mRNA expressions in the lung tissue in TNF-?-McAb group were inhibited.CONCLUSION: TNF-?-McAb inhibits the expression of iNOS and plays a role in the treatment of HPS.
