Content Determination of Noradrenaline in Plasma of Spontaneous Hypertension Rats by HPLC
- VernacularTitle:HPLC法测定自发性高血压大鼠血浆中去甲肾上腺素的含量
- Author:
Qiuyun LIANG
;
Huagang LIU
- Publication Type:Journal Article
- Keywords:
HPLC-UV methods;
Spontaneous hypertenision;
Rats;
Noradrenaline;
Content determination
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC-UV method for the content determination of noradrenaline (NA) in plasma of spontaneous hypertension rats (SHR).METHODS: The determination was performed on C18 column with mobile phase consisted of methanol-0.15 mol?L-1 potassium dihydrogen phosphate buffer (1 ∶ 9,V/V,pH 6.0) at flow rate of 1.0 mL?min-1.The detection wavelength was set at 280 nm and column temperature was room temperature.RESULTS: The linear range of NA was 0.5～40 ?g?L-1 (r=0.999 9) with an average recovery of 97.49% (RSD
