Comparison of 3 Kinds of Determination Methods for Ferrous Succinate Tablet
- VernacularTitle:琥珀酸亚铁片3种含量测定方法的比较
- Author:
Xiangde SUN
;
Li DONG
;
Rongying XI
- Publication Type:Journal Article
- Keywords:
Spectrophotometry;
HPLC method;
Cerium sulphate method;
Ferrous succinate tablet;
Content determination
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish spectrophotometry and HPLC methods for the determination of ferrous succinate tablet and to compare the results of above two methods with that of cerium sulphate method.METHODS: Ferrous reacted with 1,10-phenanthroline to form colored complex and spectrophotometry was applied to measure its absorbance.The detection wavelength was set at 510 nm.HPLC method was performed on C18 column with mobile phase consisted of 0.05 mol?L-1 KH2PO4 (pH=2.5) at detection wavelength of 215 nm.The method was quantified by an external standard mode.RESULTS: The linear range of ferrous in spectrophotometry was 0.042 44～2.546 4 mg?L-1 (r=0.999 8) with an average recovery of 99.5% (RSD
