Research on the Feasibility of Bacterial Endotoxin Test for Compound Jiagaimei Solution
- VernacularTitle:复方钾钙镁液中细菌内毒素检查法的可行性研究
- Author:
Shen CAI
;
Yingli ZHENG
;
Baoling CHEN
;
Li FANG
- Publication Type:Journal Article
- Keywords:
Compound jiagaimei solution;
Bacterial endotoxin;
Tachypleus amebocyte lysate;
Interference test;
Semi-quantitative analysis
- From:
China Pharmacy
2005;0(21):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish bacterial endotoxin test for cardioplegic solution (compound jiagaimei solution).METHODS: Semi-quantitative analysis stated in volume Ⅱ of Chinese Pharmacopoiea (2005 edition) was used to detect 3 batches of compound jiagaimei solution with tachypleus amebocyte lysate (TAL).RESULTS: The interference factors of bacterial endotoxin test could be overcome through 6-fold dilution of cardioplegic solution.The content of bacterial endotoxin in 3 batches of samples were 0.36,0.36 and 0.255 EU?mL-1,respectively.They were all lower than the limitation of bacteria endotoxin of 1.5 EU?mL-1.CONCLUSION: It is feasible to detect bacterial endotoxin of compound jiagaimei solution using semi-quantitative analysis.
