Reverse multidrug resistance in the laryngeal cancer cells by RNA interference of MDR1 gene
- VernacularTitle:MDR1基因RNA干扰逆转喉癌细胞多药耐药性的研究
- Author:
Qi ZHONG
;
Zhigang HUANG
;
Qi WANG
;
Jugao FANG
;
Xiaohong CHEN
;
Wei ZHANG
;
Hong WANG
;
Rui HONG
;
Zheng YANG
- Publication Type:Journal Article
- Keywords:
Laryngeal Neoplasms;
Drug Resistance, Neoplasm;
RNA interference;
Lentivirus
- From:
Chinese Archives of Otolaryngology-Head and Neck Surgery
2006;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE To reverse multidrug resistance(MDR)to chemotherapeutic agents in human laryngeal cancer cells(LSC-1/TAX)by knockdown the expression of P-glycoprotein(P-GP), the MDR1 gene product, using RNA interference(RNAi)technique.METHODS LSC-1/TAX cells were transfected with lentivirus vector that contains the shRNA construct targeting MDR1 mRNA.The drug resistance was measured by MTT assay in vitro, and sensitivity of the laryngeal cancer cells to various anti-neoplasm agents was quantified in vivo.The knockdown of MDR1 gene expression was assessed by immunocytochemistry in vitro and vivo.RESULTS Multidrug resistance phenotype in human laryngeal cancer cell line (LSC-1/TAX)was reversed in vitro.Tumor growth assay in vivo revealed a reverse of MDR in the laryngeal cancer cells.Immunocytochemistry showed that P-GP expression was significantly inhibited.CONCLUSION MDR1 shRNA lentivirus vectors can significantly inhibited MDR1 expression.Inhibition of MDR1 gene expression conferred an increased sensitivity of drug-resistant in laryngeal cancer cells to conventional chemotherapeutic agents.
