Obtainment and morphologic characteristics of exosomes acquired from ovarian cancer lines
- VernacularTitle:卵巢癌细胞系来源外来体的获得及形态特征观察
- Author:
Qiling LI
- Publication Type:Journal Article
- Keywords:
ovarian cancer;
immunotherapy;
exosomes;
cell line
- From:Journal of Xi'an Jiaotong University(Medical Sciences)
1982;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To obtain exosomes from the cell culture supernatants of 8910,TC-1,SKOV_(3),CAOV and 3AO ovarian cancer cell lines.Methods 8910,TC-1,SKOV_(3),CAOV and 3AO ovarian cancer cell lines were cultured separately and the cell culture supernatants were collected to seperate exosomes with multiple centrifugation and ultrafiltration.Morphology of exosomes were observed with electron microscopy.Results Exosomes were purified in 8910 and 3AO cell culture supernatants.Exosoems exhibited heterogenous round or elliptic microvesicles ranging from 30 to 90 nm in diameter with intact membrane.Exosomes were not purified in TC-1,SKOV_(3),and CAOV cell culture supernatants.Conclusion 8910 and 3AO cell lines may secrete exosomes,which are potential specific antigens for immunotherapy against ovarian cancer cells.
