Effects of Different Preparation Technologies on the Content Determination of Alkaloids in Aconitum carmichaeli by RP-HPLC
- VernacularTitle:不同样品制备方法对RP-HPLC法测定附子生物碱含量的影响
- Author:
Dabin HOU
;
Xiangsheng ZHAO
- Publication Type:Journal Article
- Keywords:
Aconitum carmichaeli;
Alkaloids;
RP-HPLC;
Sample preparation;
Separation condition
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To compare the different sample preparation and optimize the isolation of three kinds of alkaloids in Aconitum carmichaeli.METHODS:RP-HPLC was applied and performed on Zorbax extend-C18 column (150 mm?4.6 mm,5 ?m) column.The differences among the content of alkaloids prepared by preparation technologies were compared.RESULTS:The best preparation was aether ultrasonic extraction.The separation condition was as follows:mobile phase consisted of acetonitrile-0.1% ethylenediamine (gradient elution) with flow rate of 1 mL?min-1.The detection wavelength was set at 240 nm and column temperature was 30 ℃.CONCLUSION:Aether ultrasonic extraction is simple with ideal separation,extraction and re-covery to provide scientific basis for the quality control of A.carmichaeli.
