Study on Identification of Rhizoma Arisaematis and Content Determination of Flavonoids
- VernacularTitle:天南星药材的鉴别及黄酮含量测定方法研究
- Author:
Ling JU
;
Yumei CHI
;
Wei WANG
- Publication Type:Journal Article
- Keywords:
Rhizoma Arisaematis;
TLC;
Schaftoside;
Isoschaftoside;
HPLC
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the identification method of Rhizoma Arisaematis and content determination of flavon-oids.METHODS:TLC was used to identify Rhizoma Arisaematis with schaftoside and ischaftoside as reference substances.The content of flavonoids was determined by HPLC.RESULTS:TLC of test sample and that of control substance had same color dots.The linear range of schaftoside and isoschaftoside were 7.925～126.8 ?g?mL-1(r=0.999 9) and 3.996～63.94 ?g?mL-1(r=0.999 7) respectively.Average recoveries were 99.4% for schaftoside (RSD=2.10%,n=9) and 99.52% for isoschaftoside(RSD=2.42%,n=9).CONCLUSION:The method is simple,accurate and reproducible for the quality evaluation of Rhizoma Arisaematis.
