Content Determination of Asperosaponin Ⅵ in Yulin Mixture by HPLC
- VernacularTitle:HPLC法测定毓麟合剂中川续断皂苷Ⅵ的含量
- Author:
Lingling XU
;
Yuxuan ZHANG
;
Hua NIAN
- Publication Type:Journal Article
- Keywords:
Yulin mixture;
Asperosaponin Ⅵ;
HPLC;
Content determination
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish HPLC method for the content determination of asperosaponin Ⅵ in Yulin mixture.ME-THODS:The separation was performed on Zorbax EcLipse XDB-C18(150 mm?4.6 mm,5 ?m) column with mobile phase consisted of methanol-0.01 mol?L-1 hydrochloric acid (69:31) and flow rate of 1 mL?min-1.The detection wavelength was set at 212 nm and column temperature was 20 ℃.RESULTS:The linear range of asperosaponin Ⅵ was 0.022～0.23 mg?mL-1(r=0.998 9) with an average recovery of 101.33% (RSD=2.35%,n=9).CONCLUSION:The method is convenient,simple,accurate and reproducibility for the quality control of Yulin mixture.
