Content Determination of Chrysophanol in Yanbao Ⅱ Capsule by HPLC
- VernacularTitle:HPLC法测定眼保Ⅱ号胶囊中大黄酚的含量
- Author:
Peiqin ZHANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Yanbao Ⅱ capsule;
Cassia obtusifolia;
Chrysophanol;
Content determination
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a HPLC method for the content determination of chrysophanol in Yanbao Ⅱ capsule.METHODS:The separation was performed on Agilent C18(150 mm?4.6 mm,5 ?m) column with absolute alcohol-0.1% phosphoric acid (87:13) as mobile phase.The flow rate was 1.0 mL?min-1 and the detection wavelength was set at 254 nm.RESULTS:The linear range of chrysophanol was 0.042 6～0.340 8 ?g(r=0.999 9) with an average recovery of 99.39%(RSD=1.74%,n=6).CON-CLUSION:The method is simple,accurate,sensitive and reproducible for the quality control of YanbaoⅡ capsule.
