Content Determination of Shikonin in Arnebia euchroma by HPCE
- VernacularTitle:高效毛细管电泳法测定新疆紫草中左旋紫草素的含量
- Author:
Yan BAI
;
Fuhai WU
;
Bilian LUO
;
Chunli ZENG
- Publication Type:Journal Article
- Keywords:
HPCE;
Arnebia euchroma;
Shikonin;
Content determination
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the content of shikonin in Arnebia euchroma by HPCE-UV.METHODS:The separation was performed on uncoated fused silica capillary column (53 cm?50 ?m ID,46 cm effective length).2.0 mmol?L-1 H3BO3,6.0 mmol?L-1 triethylamine buffer solution and 5.0 mmol?L-1?-CD was used as background electrolyte,14 kV separation voltage and time of gravity injection 15 s (25 cm).The detection wavelength was set at 516 nm.RESULTS:The linear range of shikonin was 7.2～36.0 ?g?mL-1(r=0.999 0) with an average recovery of 100.15%(RSD=1.68%,n=6).CONCLUSION:The method is convenient,rapid,accurate and reliable for the content determination of shikonin in A.euchroma.
