Determination of Residues of Organochlorinated Pesticides in Polygonum multiflorum by GC-MS with Solid-phase Extraction
- VernacularTitle:固相萃取-GC-MS联用法测定何首乌中有机氯农药残留量
- Author:
Hongbo ZHOU
;
Zhijian FANG
- Publication Type:Journal Article
- Keywords:
Polygonum multiflorum;
Organochlorinated pesticides;
Residues;
Solid-phase extraction;
GC-MS
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To determine the residues of 9 kinds of organochlorinated pesticides in Polygonum multiflorum.ME-THODS:Samples were extracted with ethyl acetate by ultrasonic wave assistant extraction and purified on Florisil solid-phase extraction column.The residues of organochlorinated pesticides were determined with capillary gas chromatography with GC-MS.The separation was performed on DB-5MS(30 m?0.25 mm,0.25 ?m) fused-silica capillary column with injector temperature of 230 ℃.The initial column temperature was 100 ℃,then raised to 220 ℃ at the rate of 8 ℃?min-1.The column temperature was ra-ised at 250 ℃ at the rate of 5 ℃?min-1 for 10 min.The column flow was 1 mL?min-1 and injection volume was 1 ?L.RESULTS:9 kinds of organochlorinated pesticides were completely separated within 30 minutes.The average recoveries ranged from 80.4% to 97.2%(RSD ranged from 3.5% to 7.4%,n=6).CONCLUSION:The method is sensitive and accurate for the content determination of the residues organochlorinated pesticides.
