Study on Effect of the Extracts of Polygonum lapathifolium on the Activity of ?-glucosidase
- VernacularTitle:酸模叶蓼提取物对?-葡萄糖苷酶活性影响的研究
- Author:
Jinfeng WEI
;
Changqin LI
;
Xing CHANG
;
Yuxin LIU
;
Wenyi KANG
- Publication Type:Journal Article
- Keywords:
?-glucosidases;
Polygonum lapathifolium;
Extract;
Inhibitory kinetics
- From:
China Pharmacy
2005;0(19):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the effect of the extracts of Polygonum lapathifolium on the activity of ?-glucosidase.METHODS:Soxhlet extraction was applied to extract overground part of P.lapathifolium,and the inhibition effect of extracts on ?-glucosidase was determined with 96-microplate-based method,and the inhibitory kinetic characteristics of methanol extract was investigated using Lineweaver-Burk method.RESULTS:The extracts from P.lapathifolium showed good inhibitory activity.The methanol extract had the highest inhibitory activity (IC50=2.13 ?g?mL-1)followed by ethyl acetate extract (IC50=3.826 ?g?mL-1),petroleum ether extract (IC50=230.86 ?g ? mL-1).And they were all higher than that of acarbose (IC50=1 103.01 ?g?mL-1) as positive control.Inhibitory kinetics test indicated that methanol extract was noncompetitive inhibitor.CONCLUSION:The extracts of P.lapathifolium have good inhibition effect on the activity of ?-glucosidase with a good prospect of development and utilization.
