Role of prostaglandin E_2 receptor 1 in neuronal cell death induced by hypoxia on rat cortical neurocytes
- VernacularTitle:前列腺素E受体1在缺氧性神经细胞死亡中的作用
- Author:
Meiling LIU
;
Yina ZHANG
;
Lichun PEI
;
Yanqiao ZHANG
- Publication Type:Journal Article
- Keywords:
Rats;
Neurons;
Receptors,prostaglandin E
- From:
Chinese Journal of Pathophysiology
1989;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To observe the effects of prostaglandin E2 receptor1 (EP1) in neuronal cell death induced by hypoxia/reoxygenation and ischemia/reperfusion. METHODS:The cortical neurocytes of neonatal Wistar rats were cultured for 12 days and exposed to hypoxia/re-oxygenation to establish a hypoxia/re-oxygenation model. Another set of cultured primary neonatal cortical neurocytes of rats were pretreated with 17-pt (an antagonist of EP1),then underwent hypoxia for 3 h,re-oxygenated for 21 h. MTT reagent was added 1 h before measuring the cell viability. Neuron apoptosis was determined by flow cytometry. The protein expression was examined by Western blotting. RESULTS:Compared to the control cells (only underwent hypoxia /re -oxygenation and without any pretreatment),the neurons pretreated with 17-pt and then underwent hypoxia/re-oxygenation showed significantly lower survival rate (P
