Detection of circulation AFPmRNA and telomerase activity in peripheral blood mononuclear cells of patients with hepatocellular carcinoma
- VernacularTitle:肝细胞癌患者外周血甲胎蛋白mRNA与单核细胞端粒酶活性检测
- Author:
Yukun HUANG
;
Xuegong FAN
;
Fu QIU
;
Zhiming WANG
- Publication Type:Journal Article
- Keywords:
Carcinoma,Hepatocellular/diag;
alpha-Fetoproteins/blood;
Telomerase/blood;
Nest Reverse Transcriptase-PCR
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of detection of circulation alpha-fetoprotein(AFP) mRNA and telomerase activity in peripheral blood mononuclear cells(PBMC) of patients with hepatocellular carcinoma(HCC).Methods In patients with HCC,benign liver tumors,chronic liver diseases,and healthy volunteers,expression of circulation AFPmRNA and telomerase activity in PBMC were detected using nest reverse transcriptase-PCR(Nest RT-PCR) or PCR-ELISA respectively.Results(1) All the patients with benign liver tumors(n=10),chronic liver diseases(n=30),and healthy volunteers(n=30) had negative expression of circulation AFPmRNA,and the positive rate of circulation AFPmRNA in patients with HCC(73.3%,22/30)(all P
