The reversal effecy of siRNA on multidrug resistance of xenotsgraft human breast cancer tumor
- VernacularTitle:小干扰RNA逆转人乳腺癌移植瘤多药耐药的在体研究
- Author:
Xiaoming ZHUANG
;
Hong XIAO
;
Zhuo WU
;
Qingxin ZHAI
- Publication Type:Journal Article
- Keywords:
Breast Neoplasms;
Small interfering RNA;
MRP;
Multidrug Resistance
- From:
Chinese Journal of General Surgery
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the reversal effecy of small interfering RNA(siRNA) on multidrug resistance and chemotherpy sensitivity of xenografts human breast cancer tumor,and chemotherpy sensitivity in nude mice.Methods siRNA targeted on multidrug resistance protein(MRP) was transfected into MCF-7 cell xenografts tumors of mice,then the expression level of the MRPmRNA and protein was determined,and the chemotherapeutic sensitivity of the breast cancer tissues was evaluated.Results The RT-PCR and immunohistochemistry results showed that the level of MRPmRNA and protein decreased in the experimental group 24 h after the siRNA was transfected(P
