Determination of Vancomycin in Human Serum by HPLC
- VernacularTitle:HPLC法测定人血清中万古霉素的浓度
- Author:
Xinwen SONG
;
Qiong XU
;
Yang WANG
;
Huanian ZHANG
;
Hua XU
- Publication Type:Journal Article
- Keywords:
HPLC;
Vancomycin;
Serum concentration;
Pharmacokinetics
- From:
China Pharmacy
2005;0(18):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish an HPLC method for the determination of the concentration of vancomycin in human serum. METHODS: Serum samples were centrifuged after serum protein was precipitated by 20% metaphosphoric acid. The separation was performed on Hypersil C18 column with mobile phase consisted of acetonitrile-0.012 5 mol?L-1 potassium dihydrogen phosphate buffer (10 ∶ 90) at flow rate of 0.8 mL?min-1. The detection wavelength was set at 236 nm and column temperature was set at 25 ℃. RESULTS: The average relative recovery rate of vancomycin in low, medium and high concentrations (2.5,30.8,150.4 mg?L-1) were 99.8%, 101.1% and 98.9% respectively. The inter-day and intra-day RSD were less than 2.5%(n=5). The limit detection of vancomycin in serum was 2.0 mg?L-1. The linear range was within 2.0～170.0 mg?L-1(r=0.999 8). CONCLUSION: The method is simple, accurate, and rapid for the monitoring and phamacokinetic studies of vancomycin in human serum.
