Protection Effect of Recombinant Human Growth Hormone for Injection on Ischemic-type Biliary Lesion in Rats
- VernacularTitle:注射用重组人生长激素对缺血型胆道病变模型大鼠的保护作用研究
- Author:
Zheng WANG
;
Jie ZHOU
;
Yu WANG
;
Yixiong LIN
- Publication Type:Journal Article
- Keywords:
Liver transplantation;
Ischemic-type biliary lesion;
Rats;
Recombinant human growth hormone
- From:
China Pharmacy
2005;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To investigate the changes of ultrastructure of biliary epithelia with ischemic-type biliary lesion (ITBL) and the protection effect of exogenous growth hormone (GH) in order to provide experimental information for prevention of biliary complication after liver transplantation. METHODS: Rats underwent liver transplantation were randomized into control group, ITBL group and recombinant human growth hormone (rhGH) group (n=6, respectively). Each group was given drugs at the same day postoperation. 7 days later, the specimen of bile ducts was collected. The epithelial changes of biliary tract were observed with TEM, and the injury degree of the epithelia was analyzed quantitatively in a specialized image analysis system. The epithelial plane measurement parameters of each group were measured, including the density of area and quantity of epithelia, and the density of area of epithelial microvilli. RESULTS: As compared with control group, in ITBL group the integrity of bile ducts was destroyed, epithelial microvilli became sparse, the epithelial plane measurement parameters were significantly decreased (P
