Determination of Peroxide Value in Hydroxycamptothecin Freeze-dried Liposome
- VernacularTitle:羟基喜树碱冻干脂质体中过氧化值的测定
- Author:
Shixia GUAN
;
Haigang LI
;
Can PENG
;
Zhongze LIN
- Publication Type:Journal Article
- Keywords:
Peroxide value;
Liposome;
UV spectrophotometry;
Determination
- From:
China Pharmacy
2005;0(17):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish a method for the determination of the peroxide value of hydroxycamptothecin freeze-dried liposome. METHODS: Colorimetric method with ferric thiocyanate and UV spectrophotometry were adopted for the content determination of peroxide value at detection wavelength of 500 nm. RESULTS: The linear range of iron was 0.2～4 ?g?mL-1 (r=0.999 0) with an average recovery of 99.2% (RSD=1.2%, n=9). The peroxide value of 3 batches of hydroxycamptothecin freeze-dried liposome was 0.48,0.45,0.43 mmol?kg-1. CONCLUSION: The method is sensitive and precise for the determination of the peroxide value of liposome.
