Effect of Processing Method on the Content of 3 Kinds of Alkaloids in Phellodendron chinense
- VernacularTitle:炮制对黄柏中3种生物碱含量的影响
- Author:
Yajie WEI
;
Mingquan XIE
- Publication Type:Journal Article
- Keywords:
Phellodendron chinense;
Processed product;
Alkaloid;
HPLC;
Content
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the influence of processing method on the content of 3 kinds of alkaloids in Phellodendron chinense. METHODS:HPLC was used to determine the content of 3 kinds of alkaloids in P. chinense,such as stir-frying with wine,salt-roast process and carbonization. The separation was performed on Zorbax SB-C18(250 mm?4.6 mm,5 ?m) column. The mobile phase consisted of acetonitrile-0.1% phosphoric acid (48 ∶ 52,0.1 g SDS per 100 mL) with flow rate of 1.0 mL?min-1. Detection wavelength was set at 265 nm and column temperature was set at room temperature. Injection volume was 10 ?L. RESULTS:Except for special case,the content of berberine hydrochloride,pamatine hydrochoride and jateorizine hydrochoride were all decreased after processing,especially carbonization.CONCLUSION:The 3 kinds of alkaloids can be also used as index of quality control of processed P. chinense. The method is simple,rapid and accurate for the determination of 3 kinds of alkaloids in P. chinense.
