Effect of Fuyuan Capsule on the Expression of HIF-1? Kidney of Sepsis Rats
- VernacularTitle:复原胶囊对脓毒症大鼠肾组织HIF-1?表达的影响
- Author:
Jian MAO
;
Rongheng LI
- Publication Type:Journal Article
- Keywords:
Fuyuan capsule;
Sepsis;
Kidney;
HIF-1?
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To explore the effect of Fuyuan capsule on the mRNA expression of HIF-1? in kidney of sepsis rats. METHODS:Male SD rats were randomly divided into sham group,model group and Fuyuan capsule group (low dose and high dose) with 4 rats in each group. CLP operation was done in Fuyuan capsule groups after four days of intragastric administration. RT-PCR testing was applied to determined the expression of HIF-1a in 3 h,6 h,12 h and 24 h after model induction. The level of IL-6 was measured by ELISA method. Blood of heart was sampled for detection of renal function. HE staining was used to observe pathological change of kidney. RESULTS:As compared with sham group,the mRNA expression of HIF-1? increased gradually within 3 h and reached peak at 24 h(P
