Simultaneous Determination of 4 Kinds of Flavonoids in Radix Astragali by HPLC
- VernacularTitle:HPLC法同时测定黄芪中4种黄酮类成分的含量
- Author:
Lijuan LIANG
;
Kuijun ZHAO
- Publication Type:Journal Article
- Keywords:
Radix Astragali;
Calycosin-7-O-?-D-glycoside;
Ononin;
Calycosin;
Formononetin;
HPLC;
Content
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To develop an HPLC method for simultaneous determination of calycosin-7-O-?-D-glycoside,ono-nin,calycosin and formononetin in Radix Astragali from eleven different habitats and to explore the internal factors of geoherbalism based on aspect of contents of active constituents. METHODS:The sample was separated on Zorbax Eclipse XDB-C18(250 mm?4.6 mm,5 ?m)column. The mobile phase consisted of acetonitrile-0.2% formic acid solution (gradient elution). The UV detection wavelength was set at 280 nm. RESULTS:The linear ranges of calycosin-7-O-?-D-glycoside,ononin,calycosin and formononetin were 0.005 1～0.510 mg?mL-1,0.005 0～0.300 mg?mL-1,0.004 9～0.294 mg?mL-1,0.004 6～0.276 mg?mL-1,respectively(r=0.999 9).Calycosin-7-O-?-D-glycoside and ononin took up a big proportion in Radix Astragali from Inner Mongolia and Shanxi; calycosin and formononetin took up a big proportion in Radix Astragali from Anguo,Chicheng of Hebei province and Dingxi of Gansu province. CONCLUSION:The method is simple,rapid and accurate. Results of study are in line with textual research on Radix Astragali.
