Study on Chemical Constitutes of Ligusticum chuanxiong and Content Determination of Ligustilide
- VernacularTitle:川芎化学成分分离鉴定与藁本内酯的含量测定
- Author:
Ling ZHANG
;
Youping LIU
;
Min LI
;
Huiyong LI
;
Jingxian FAN
- Publication Type:Journal Article
- Keywords:
Ligusticum chuanxiong;
Ligustilide;
Extraction;
Isolation;
Quality control
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the chemical constitutes of Ligusticum chuanxiong and to establish the method for the content determination of ligustilide. METHODS:The compounds were extracted and percolated by ethanol. Then the samples were separated using silica gel and identified by 1H-NMR,13C-NMR data.HPLC was used to assay the contents of ligustilide. RESULTS:Three compounds were isolated and their structures were identified as Z-ligustilide,Z-6,8',7,3'-diligustilide and Z,Z'-6,6',7,3'a-diligustilide. The contents of ligustilide were no less than 0.70%.CONCLUSION:The separation method can be used to prepare high-purity ligustilide reference substance. And the determination method can be used for quality control of L. chuanxiong.
