Specific serum protein of colorectal cancer screened by fingerprint
- VernacularTitle:采用蛋白指纹图谱技术筛选大肠癌特异性相关蛋白
- Author:
Jungang LIU
;
Feng GAO
;
Sen ZHANG
;
Chuanyuan LIU
- Publication Type:Journal Article
- Keywords:
Colorectal Neoplasms;
Biomarker;
Protein Chip;
Surface Enhanced Laser Desorption/Ionization Time-of-Flight Mass Spectrometry(SELDI-TOF-MS)
- From:
Chinese Journal of General Surgery
1997;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the difference of proteomic spectra in serum of patients with colorectal cancer(CRC) in order to build a proteomic pattern and find a method for early diagnosis of CRC.Methods We screened for potential tumor biomarkers of serum samples from 48 CRC patients and 34 healthy subjects by using CM10(Ciphergen Company,USA) and the technology of Surface enhanced laser desorption/ionization time-of-flight mass spectrometry(SELDI-TOF-MS).Using Ciphergen Protein Chipsoftware 5.1,a proteomic pattern was constructed.The constructed pattern was then tested by an independent set of masked serum samples from 33 colorectal cancer patients and 34 healthy subjects.Results(1) The contents of 27 proteins in the CRC to healthy subjects groups were significantly different(P
