Study on Integron of AmpC Enzyme-producing Escherichia Coli
- VernacularTitle:产AmpC酶大肠埃希菌中整合子的研究
- Author:
Sijing LU
- Publication Type:Journal Article
- Keywords:
Integron;
AmpC enzyme-producing Escherichia coli;
Gene cassettes;
PCR
- From:
China Pharmacy
2005;0(14):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To investigate the classification,structure and integron-mediated AmpC enzyme gene transfer of integron in 25 strains of AmpC enzymeproducing Escherichia coli. METHODS:Sensitivity of test strains to 20 kinds of antibiotics was tested by microdilution method. PCR and sequencing were performed on test strains to identify integrase gene(intI)and its location,the product of variable region of positive integrons(Int)respectively. RESULTS:Class ⅠintI were indentified in 20 strains (80%)of 25 strains Escherichia coli. The most drug resistance box cassettes were aadA5 and dfr17 and integory encoding AmpC gene cassette was not observed. CONCLUSION:Class Ⅰ integron resided in AmpC enzyme-producing Escherichia coli widely. The cause of drug resistance of integorn to aminoglycosides,sulfonamides,chloramphenicol is drug resistance gene cassette. Gene cassette does not play important role in integron-mediated antibiotic resistant gene transformation.
