Determination of EPA and DHA in Ray Oil by HPLC
- VernacularTitle:HPLC法测定魟鱼鱼油中二十碳五烯酸、二十二碳六烯酸的含量
- Author:
Hongwei PENG
;
Bin GUO
- Publication Type:Journal Article
- Keywords:
Ray;
EPA;
DHA;
HPLC method;
Content determination
- From:
China Pharmacy
2005;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish a method for the determination of EPA and DHA in ray oil.METHODS:The determination was carried out on Diamonsil C18 column.The mobile phase consisted of acetonitrile-water(95 :5) with flow rate of 1.0 mL?min-1 and detection wavelength set at 220 nm.The column temperature was set at 25 ℃.RESULTS:The linear ranges were 1～4 ?g for EPA(r=0.999 1) and 2～8 ?g for DHA(r=0.999 4).The average recoveries were 99.23% for EPA(RSD=0.49%) and 99.30% for DHA(RSD=0.27%).CONCLUSION:The method is simple,rapid,accurate and reproducible for the detection of EPA and DHA in ray oil.
