Content Determination of Total Alkaloids in Cultivated BulBus Fritillariae Cirrhosae by Spectrophotograhpy
- VernacularTitle:分光光度法测定栽培川贝母中总生物碱的含量
- Author:
Jiangping YU
- Publication Type:Journal Article
- Keywords:
Cultivation;
BulBus Fritillariae Cirrhosae;
Spectrophotography;
kashmirine;
Total alkaloid;
Content determination
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for the determination of total alkaloid in cultivated BulBus Fritillariae Cirrhosae. METHODS:Spectrophotography was used to determine the content of total alkaloids with kashmirine as control,bromocresol green as colored indicator at detection wavelength of 416 nm. RESULTS:The linear range of kashmirine were 4.5～14.5 ?g?mL-1 (r=0.999 6) with an average recovery of 98.76%(RSD=2.5%,n=6).CONCLUSION:The method is simple,accurate and relia-ble for the quality control of cultivated BulBus Fritillariae Cirrhosae.
