Determination of Anticancer Activity Components of Flavonoids from Hedyotis diffusa by HPLC
- VernacularTitle:白花蛇舌草中黄酮类抗肿瘤活性成分的HPLC分析
- Author:
Zhenzhong SHAO
;
Xiaobin JIA
;
Ran XIN
;
Xiaoyong JIN
;
Yan CHEN
- Publication Type:Journal Article
- Keywords:
Hedyotis dippusa;
Quercetin;
kaempferol;
HPLC;
Content determination
- From:
China Pharmacy
2005;0(15):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To establish the method for the simultaneous determination of anticancer activity components of flavonoids from Hedyotis diffusa,i.e. quercetin,kaempferol. METHODS:HPLC was applied to determine the contents and performed on Alltima C18(250 mm?4.6 mm,5 ?m) column. Mobile phase consisted of methanol(A)-0.5% glacial acetic acid,(gradient elution). The detection wavelength was aet at 350 nm. RESULTS:The linear range of quercetin was 0.006 2～0.244 0 ?g(r=0.999 8)and that of kaempferol 0.007 8～0.310 6 ?g(r=0.999 9). The average recovery of quercetin was 101.84%(RSD=1.79%,n=6) and that of kaempferol 99.04%(RSD=2.90%,n=6). CONCLUSIONS:The method is simple,accurate and reproducible for the quality control of H. diffusa.
