Preliminary Study on the Safety of Polylatic Acid as Brain Implants Carrier
- VernacularTitle:聚乳酸作为脑植入剂载体的安全性初步研究
- Author:
Huafei TANG
;
Songqing LIU
- Publication Type:Journal Article
- Keywords:
Polylactic acid;
Neurons;
CCK-8;
Apotosis;
Safety
- From:
China Pharmacy
2005;0(13):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE:To study the safety of polylatic acid used as sustained-release drug carrier in brain.METHODS:The toxicity and apoptosis of in vitro primary cultured cortical neurons in rats were evaluated using CCK-8 and Hoechst33258 staining after treated with polylatic acid and compared with the normal control group.Self-made polylatic acid blank tablet was implanted into rat brain and after 30 days the neuronal function was assessed through behavior ability testing,HE staining and Nissl staining,compared with sham groups and normal control group.RESULTS:When neurons were cultured for 7,9 and 12 days in polylatic acid group,there was no significant difference in absorbance value of CCK-8 and apoptosis rate between normal control group and polylatic acid group.Light inflammatory was observed in rat brain tissue in polylatic acid blank tablet group after 30 days,as compared with sham group.But the morphology,number and function of neuron had no obvious change.CONCLUSION:Polylactic acid has no significant inhibitory and inducing apoptosis effect on in vitro cultured cortical neurons and also has no obvious toxicity on brain tissue.
