Determination of Main Active Components in Compound Polygonum cuspidatum Semisolid Matrix Capsule
- VernacularTitle:复方虎杖半固体骨架胶囊中主要有效成分含量测定方法研究
- Author:
Ruizhi ZHAO
- Publication Type:Journal Article
- Keywords:
Compound Polygonum cuspidatum semisolid matrix capsule;
Content determination;
Emodin;
Resveratrol;
Polydatin;
Gentiopicrin
- From:
China Pharmacy
2001;0(11):-
- CountryChina
- Language:Chinese
-
Abstract:
OBJECTIVE: To establish the method for the determination of emodin, resveratrol, polydatin and gentiopicrin in the Compound Polygonum cuspidatum semisolid matrix capsule in order to provide basis of its quality control. METHODS: TLC scanning method was used to determine emodin and resveratrol at same plate and the developer was petroleum ether-butyl acetate-methanol-glacial acetic acid(4∶1∶0.7∶0.03) with determination wavelength of 285 nm and 293 nm respectively. Polydatin and gentiopicrin were determined at same plate and the developer was chloroform-methanol(7 ∶ 2)with determination wavelength of 313 nm and 270 nm respectively. RESULTS: All target components were separated and the standard curves of emodin, resveratrol, polydatin and gentiopicrin showed good linearity. Average recoveries of them were 100.1%(RSD=0.86%),99.6%(RSD=2.86%), 101.4%(RSD=0.99%),98.5%(RSD=2.89%),respectively. CONCLUSION: Established method is rapid, simple for the quality control of Compound P. cuspidatum semisolid matrix capsule.
